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KMID : 0376419830070020255
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Chonbuk University Medical Journal
1983 Volume.7 No. 2 p.255 ~ p.266
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Effects of Cromolyn Sodium on the Mastocalcergy
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Abstract
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In order to study the effect of cromolyn sodium on the mastocalcergy in the rats and the role of the mast cells in this process, lead acetate and compound 48/80 were injected into the rats with or without pretreatment of cromolyn sodium. Histochemical and electron microscopic studies were carried out on the back skin taken from´ the rats - which were sacrified at various time intervals after the injection of lead acetate and compound 48/80.
The following conclusions were obtained ;
1. The back skin of the rats at 1 day after intravenous injection of lead acetate followed by immediate subcutaneous injection of compound 8.8/80 showed calciphylactic wheal, about 4.6 x 5.5cm in diameter at the injected area of compound 48/80. The number of the mast cells in the back sk_a was shown to be less than that of the control abdominal skin at each time interval, and decreased, gradually and significantly, as the time lapsed. Calcification of the mast cell granules was not°d at 5 hours, and this calcified mast cell granules were disintegrated into dust-like fine precipitates on adjacent collagenous fibers at 1 day, ard resulted in petrification at 6 days.
On electron microscopy of the back skin from experimental rats fine dense particles, considered to be calcium salts, was noted to be abundant around the mast cell granules at 5 hours, and also abundant in the vacuoles of the mast cell, and few in the mast cell granules and on adjacent collagenous fibers at 1 day.
2. The back skin of cromolyn sodium pretreated rats showed calciphylactic wheal, about 2.6 x 3.5cm in diameter, at the injected area of compound 48/80 at 1 day. The number of the mast cells showed no significant changes ccmparing with that of the control skin up to 5 hours but showed significant difference at each time interval as ccmFared with those of cromolynodium non-pretreated rats. At the light microscopic level calcification of the mast cell granules and defosition of calcium salts on the collagenous fibers were not detectable at 5 hours but were noted at 1 day.
Electron microscopy revealed the presence of few fire particles in the mast cell granules and still fewer on the collagencus fibers both 5 hours and 1 day after the experimental procedures.
From the above results it is suggested that mastocalcergy may be partially prevented by pretreating with crcmolyn sodium in the rats aid dhposition of calcium salts in and around the mast cell granules and on the adjacent collagenous fibers indicate that the mast cell gra pules may play some key roles in calcification and mineralization of soft tissue.
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